rabbit antibody s100 β  (Proteintech)

Journal: Journal of Clinical Pathology

Article Title: Unusual fusion gene rearrangements in patients with nodular fasciitis: a study of rare and novel USP6 fusion partners with a review of the literature

doi: 10.1136/jcp-2023-208768

Figure Lengend Snippet: Patient characteristics and clinical findings in USP6::MYH9 fusion cases

Article Snippet: Thin histological sections (3 μm thick) were used, and each sample was stained using the following antibodies and protocols: anti-smooth muscle actin (SMA) mouse monoclonal antibodies (clone 1A4, BioSB— Bioscience for the World, dilution 1:75; pretreatment: heating up to 99°C in a pH 6 buffer in a water bath); anti-H-caldesmon mouse monoclonal antibodies (clone BSB-19|, BioSB, dilution 1:100; pretreatment: heating up to 99°C in a pH 9 buffer in a water bath); anti-desmin mouse monoclonal antibodies (clone D33, BioSB, dilution 1:100; pretreatment: heating up to 99°C in a pH 9 buffer in a water bath); anti-S100-β rabbit monoclonal antibodies (clone EP32, BioSB, dilution 1:300; pre-treatment: heating up to 99 ◦C in a pH 9 buffer in a water bath); anti-Ki-67 mouse monoclonal antibodies (clone MIB-1, BioSB, dilution 1:150; pretreatment: heating up to 99°C in a pH 6 buffer in a water bath).

Techniques:

Journal: Regenerative Biomaterials

Article Title: Anisotropic microtopography surface of chitosan scaffold regulating skin precursor-derived Schwann cells towards repair phenotype promotes neural regeneration

doi: 10.1093/rb/rbae005

Figure Lengend Snippet: Characterization of SKPs and SKP-derived SCs. ( A ) Cultured SKPs showed floating spherical clone morphology. ( B ) SKP-derived SCs displayed long-spindle shape and side-by-side alignment. ( C ) GFP (green)-positive SKP-SCs showed expression of SC marker S100-β (red) with Hoechst (blue) labeled cell nuclei. Scale bar, 50 μm.

Article Snippet: GFP-positive SKP-SCs were fixed with 4% paraformaldehyde, and blocked, then incubated with rabbit anti-S100-β primary antibody (1:400, ThermoFisher Scientific, Carlsbad, CA, USA) overnight at 4°C, followed by reaction with goat anti-rabbit-IgM-cyanine 3 (Cy3) (1:400, Abcam, Cambridge, UK) and Hoechst 33342 (Abcam) counterstaining.

Techniques: Derivative Assay, Cell Culture, Expressing, Marker, Labeling

Journal: Regenerative Biomaterials

Article Title: Anisotropic microtopography surface of chitosan scaffold regulating skin precursor-derived Schwann cells towards repair phenotype promotes neural regeneration

doi: 10.1093/rb/rbae005

Figure Lengend Snippet: The SKP-SC-secretome from ‘30 μm’ surface group promoted primary SCs proliferation and migration. ( A ) Primary SCs showed positive expression of SC marker S100-β (red) with Hoechst labeled nuclei (blue). scale bar, 100 μm. ( B ) Representative images of EdU (red) labeled nuclei with Hoechst labeled nuclei (blue) background of SCs treated with ‘30 μm-CM’, ‘flat-CM’, and ‘MN-medium’ for 24 h, respectively. Scale bar, 100 μm. ( C ) Representative images of SCs scratch healing showed different migration area ratio of SCs in three groups after treatment for 8 h. Scale bar, 100 μm. ( D ) Statistical analysis showing the percentage of EdU positive SCs in ‘30 μm-CM’ group were remarkedly more than that in ‘flat-CM’ and ‘MN-medium’ group. ( E ) Statistical analysis showing the wound healing percentage of SCs in ‘30 μm-CM’ group were significantly higher than that in ‘flat-CM’ and ‘MN-medium’ group. n = 3, *P < 0.05, **P < 0.01.

Article Snippet: GFP-positive SKP-SCs were fixed with 4% paraformaldehyde, and blocked, then incubated with rabbit anti-S100-β primary antibody (1:400, ThermoFisher Scientific, Carlsbad, CA, USA) overnight at 4°C, followed by reaction with goat anti-rabbit-IgM-cyanine 3 (Cy3) (1:400, Abcam, Cambridge, UK) and Hoechst 33342 (Abcam) counterstaining.

Techniques: Migration, Expressing, Marker, Labeling

Journal: PLoS ONE

Article Title: Satellite glial cells modulate cholinergic transmission between sympathetic neurons

doi: 10.1371/journal.pone.0218643

Figure Lengend Snippet: (a-b) Representative confocal images of (a) P2 and (b) P21 SCG. Sympathetic neurons were stained for the neuron-specific marker MAP2 (red), satellite glial cells for the glial cell marker S100β (green) and cell nuclei using DAPI (blue). Scale bar = 100 μm. (c-d) Quantification of (c) neuron soma size, measured as average cell area in the section and (d) neuronal and glial cell densities from sections of P2 (n = 3; mean ± s.e.m.) and 3 wks (n = 3; mean ± s.e.m.). ***p<0.001, **p<0.01, *p<0.05 determined by ANOVA followed by pairwise post hoc (Tukey’s HSD) comparison test.

Article Snippet: They were then incubated overnight with primary antibodies at the following concentrations: chicken anti-Microtubule Associated Protein 2 (MAP2) polyclonal antibody (Sigma-Aldrich, EMD Millipore, Darmstadt, Germany, AB5543, 1:1000) and rabbit anti-S100 calcium-binding protein B β-subunit (S100-β) polyclonal antibody (Agilent Dako, Santa Clara, CA, USA Z0311, 1:400).

Techniques: Staining, Marker

Journal: PLoS ONE

Article Title: Satellite glial cells modulate cholinergic transmission between sympathetic neurons

doi: 10.1371/journal.pone.0218643

Figure Lengend Snippet: Satellite glial cells partly prevent sympathetic neuronal death upon NGF deprivation. (a-b) Establishment of sympathetic neuron-satellite glia co-cultures. Neurons (N) were cultured alone (a) or in the presence of satellite glia (b) in the presence of 5 ng/ml NGF in serum-containing medium. Cultures were fixed at 12 days in vitro (div) and stained for Tuj-1 (neuronal marker, in red), S100β (glial cell marker, in green) and DAPI (nuclear staining, in blue). Scale bar represents 50 μm. Under these growth conditions, glia did not alter the number of sympathetic neurons (c). (d) Neurons alone (N), (e) Neurons and glia (N+G), and (f) N+G with anti-NGF antibody (1:1000, final concentration 1 μg/ml). (g) Quantification of cell survival upon NGF deprivation. Data are shown as percent neuronal survival compared to comparable cultures (neurons alone or neurons + glia) grown in the presence of 5 ng/ml NGF in serum-free medium (n = 3 independent cell culture experiments, One-way ANOVA, ***p<0.001). All data are represented as mean ± s.e.m.

Article Snippet: They were then incubated overnight with primary antibodies at the following concentrations: chicken anti-Microtubule Associated Protein 2 (MAP2) polyclonal antibody (Sigma-Aldrich, EMD Millipore, Darmstadt, Germany, AB5543, 1:1000) and rabbit anti-S100 calcium-binding protein B β-subunit (S100-β) polyclonal antibody (Agilent Dako, Santa Clara, CA, USA Z0311, 1:400).

Techniques: Cell Culture, In Vitro, Staining, Marker, Concentration Assay